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Information on approval of the analysis by various committees charged with monitoring study involving human topics is supplied in the Supplementary Appendix. Vaccines A reconstituted dosage of PsA-TT vaccine as adjuvant, TRIS buffer, 0.01 percent thimerosal, and 0.9 percent sodium chloride. A reconstituted dose of reference vaccine, PsACWY , a week and 4 weeks following the booster vaccination. For every blood sample collected, a thicker smear was examined for malaria. Measurement of SBA titers was performed at the ongoing health Protection Agency, Manchester, UK,20 and the ELISA was performed at the Centers for Disease Control and Avoidance , Atlanta, by using the standard reference serum CDC1992.21 The SBA reference strain was F8238, and titers were expressed as the reciprocal of the final serum dilution, producing a colony-count reduction of at least 50 percent after 60 minutes of incubation.